seeblue protein ladder Ctbp1 western blot

Seeblue protein ladder Ctbp1 western blot series#

CTBP1 divergent transcript (CTBP1-AS2), as a newly identified lncRNA, was limitedly reported in cancers. For instance, linc00511 is highly expressed in CC and knockdown of linc00511 dampens CC cell proliferation and reduces drug resistance to paclitaxel. In CC, some lncRNAs were discovered to be aberrantly expressed and exerted important biological functions. To date, the pathologic roles of most lncRNAs remain unknown, which indicates the extensive potential of lncRNAs in the prediction and treatment of various cancers. Furthermore, lncRNAs have been identified as novel biomarkers of many cancers.

Seeblue protein ladder Ctbp1 western blot series#

Dysregulation of lncRNAs is associated with a series of biological processes, such as cell proliferation, apoptosis, invasion and migration. Recent findings indicated that lncRNAs play vital roles in gene regulation at the transcriptional level. Long non-coding RNAs (lncRNAs) are a class of RNAs longer than 200 nucleotides but lack the protein-coding potential. Therefore, it is essential to explore the molecular mechanisms behind the initiation and development of CC. At present, radiotherapy chemotherapy and surgery remain the main clinical therapeutic methods for patients with CC. Preclinical models have been used for the treatment of CC patients. Global strategies for the prevention and screening of CC remain to be improved based on various geographic settings and health systems. Recently, an increasing trend of morbidity and mortality of CC has been discovered in China. Based on cancer statistics in 2019, there were 13,170 estimated new cases and 4250 estimated deaths in the United States. Human papilloma virus (HPV) is the major cause for the high risk of CC.

Each year, more than 500,000 cervical cancer cases are diagnosed and approximately 300,000 patients die of cervical cancer worldwide. ConclusionĬTBP1-AS2 regulates CC progression via sponging miR-3163 to up-regulate ZNF217.Ĭervical cancer (CC) is the fourth most common diagnosed cancer and the fourth leading cause of cancer-related deaths in females globally. ZNF217 up-regulation abrogated the tumor suppressing effects of CTBP1-AS2 knockdown. CTBP1-AS2 acted as a miR-3163 sponge to elevate ZNF217 expression. Additionally, Zinc finger protein 217 (ZNF217) was identified as a direct target of miR-3163. MiR-3163 inhibition abolished the anti-tumor effects of CTBP1-AS2 knockdown. Cytoplasmic CTBP1-AS2 was found to be a miR-3163 sponge in CC cells. CTBP1-AS2 facilitated xenograft tumor growth in vivo. Knockdown of CTBP1-AS2 curbed cell proliferation, migration and invasion, while stimulated cell apoptosis in vitro. ResultsĬTBP1-AS2 was significantly overexpressed in CC cell lines. Luciferase reporter, RNA pull down and RIP assays were performed to determine the specific mechanical relationship between CTBP1-AS2, miR-3163 and ZNF217. In vivo animal study was utilized to investigate the role of CTBP1-AS2 in tumor growth. In vitro functional assays, including CCK8, EdU, TUNEL and transwell assays were applied to explore the functions of CTBP1-AS2 in CC cell proliferation, apoptosis and migration. QRT-PCR and western blot assays were used to detect relevant RNA and protein expressions. This paper was aimed at exploring the role of CTBP1 divergent transcript (CTBP1-AS2) in cervical cancer (CC) progression. Compatible with chemiluminescent substrates and fluorescent secondary antibodies.Long non-coding RNAs (lncRNAs) play significant roles in tumorigenesis and can contribute to identification of novel therapeutic targets for cancers. Western blotting: detection of the two unstained bands via the detection method used for the target protein.Western blot confirmation-detect the two unstained proteins (30 kDa and 80 kDa) using the detection system for the target protein.Fluorescent visualization-detect the 10 stained proteins using the 670 nm red laser or 700 nm channel of a fluorescence imager.The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use.Ĭompare and view all other protein standards and ladders › IBright Prestained Protein Ladder contains twelve recombinant proteins, ten (11 to 250 kDa) that are blue-stained and fluor-labeled for direct and near-IR fluorescent visualization and protein sizing, and two (30 kDa and 80 kDa) that are unstained and contain IgG binding sites to bind the primary and secondary antibodies used for chemiluminescent and fluorescent detection of the target protein.